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KMID : 0380020070220010058
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Korean Journal of Biotechnology and Bioengineering
2007 Volume.22 No. 1 p.58 ~ p.61
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Insecticidal Effect of Dermatoohagoides pteronyssinus using Ginkgo biloba Leaves Extracts
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Lee In-Hwa
Park Ju-Young
Choi Seung-Hyun
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Abstract
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A Insecticidal effect of Ginkgo biloba leaves extract was conducted for Dermatophagoides pteronyssinus as a predominant species in korea. D. pteronyssinus has been cultured in constant temperature and humidity chamber at the 25 and 75% of relative humidity. The mortality of mites was determined by counting the dead bodies for every hour within 48 hours with pin hall microscope after treated by Ginkgo biloba leaves extract spreaded on 0.1 g of mass cultured media. The sequence of mortality for D. pteronyssinus are as follows, bilobalide was 91.6%, 80 water extract was 82.8%, second water fraction was 75%, ethyl acetate fraction from 80 water fraction was 73%, first ethyl acetate fraction from 80 water extract was 69.4%, putaltrin was 65%, distilled water was 58%, methanol extract was 57.8%, Ginkgolide-A was 57.1 %, ethyl acetate fraction of 80 water extract was 55%, respectively. From the these results we conclude that the bilobalide is the most effective component in the Ginkgo biloba leaves extract having insecticidal effect on house dust mite.
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KEYWORD
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Ginkgo biloba leaves extract, house dust mite, Dermatophagoides pteronyssinus, insecticidal effect
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